Changes between Version 1 and Version 2 of Courses/DataIntegration

Timestamp:

2014-09-18T12:25:18+02:00 (10 years ago)

Author:

Pieter Neerincx

Comment:

--

Courses/DataIntegration

@@ -11 +11 @@
 == Verify exome capturing kit annotation ==
 
-If you are looking for genetic variation in human protein coding genes, sequencing the whole genome is not economical as the human exome as only ~ 2% of the genome. To enrich the NGS analysis you can order capturing kits for your genes of interest. The latest version of the most popular exome capturing kit is the SureSelect All Exon capturing kit version 5 from Agilent. Will your genes of interested be covered completely and captured well with this kit? In other words what was the manufacturer's definition of ''"whole Exome"'' at the time of the design of this kit?
+If you are looking for genetic variation in human protein coding genes, sequencing the whole genome is not economical as the human exome as only ~ 2% of the genome. To enrich the NGS analysis you can order capturing kits for your genes of interest. The latest version of the most popular exome capturing kit is the !SureSelect All Exon capturing kit version 5 from Agilent. Will your genes of interested be covered completely and captured well with this kit? In other words what was the manufacturer's definition of ''"whole Exome"'' at the time of the design of this kit?
 
 To check this we will:
@@ -20 +20 @@
  * Click the ''Configure this page'' button
  * In the popup window select the 4th tab ''Personal Data''
- * Go to ''Custom data'' -> ''Add your data'' in the column on the left and upload the *.bed file for the SureSelect All Exon V5 kit.
+ * Go to ''Custom data'' -> ''Add your data'' in the column on the left and upload the *.bed file for the !SureSelect All Exon V5 kit.
  * Click the checkmark at the top-right corner of the popup window to close it and wait for the display to update.
  * Optionally click ''Configure this page'' again and enable/disable other annotation tracks
  * Inspect your gene of interest: does the capturing kit capture all exons completely?
+
+== Verify custom capturing kit annotation==
+
+For a more targeted approach we designed a capturing kit for all genes from the Clinical Genomics Database (CGD). Upload the *.bed file for this capturing kit too and check if this more recent kit is better for your genes of interest. If your gene of interest was not part of the CGD, you can 
+
+== BioMart ==